New biosensor generation based on high density protein microarrays and infrared microscopy/imaging

Abstract : Proteins are not only involved in a very large number of biological processes but are also more and more widely used in the field of health, nanotechnologies and bio-technologies. In turn, there is an urgent need to develop approaches for (i) fast identification and structural characterization of a large panel of proteins and (ii) their selective identification in complex media. More publication, see Sort for gooormaghtigh for the topic described above The first part of the project aims at building high density biosensors able to monitor quickly the presence of a large variety of proteins in a solution. A new approach based on FTIR imaging will detect binding of proteins on high density microarrays of receptors (e.g. hundreds of specific antibodies) in a single measurement. Full FTIR spectra will be obtained for each species, bringing information on protein identity, post-translational modifications and conformation. The unique advantage of this technique is that the infrared spectrum of the protein is used for both detection and analysis of structure/PTN,… There is therefore no need to use fluorescent reporters and allows the exploitation of the huge amount of information present in the infrared spectrum The elaboration of the various biosensors will rely on an original surface functionalization strategy that will be developed in collaboration with the organic chemistry department of ULB. Mo
Promoteur/Supervisor : Prof. Goormaghtigh Erik
Email :
Site Web/Web site :
Centre de recherche/Research center : Center for Structural Biology and Bioinformatics
Faculté/Faculty : Faculty of Sciences/Faculté des Sciences
Ecole doctorale/Graduate Colleges : Science/Sciences
Ecole doctorale thématique/Graduate School (French Only): Structure et fonction des macromolécules biologiques, bioinformatique et modélisation (SFMBBM)

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