Live imaging study of the cytoskeleton dynamics allowing cell migration during morphogenesis of the mouse embryo.

Abstract : Most morphogenetic migration events rely on collective rather than individual migration. This poorly characterized migration mode is widely used in adulthood, for regeneration or dissemination of cancer cells. The embryo provides a perfect frame to study collective migration in a physiological 3D environment. I showed that in the mouse embryo, anterior visceral endoderm (AVE) migration occurs within an epithelium: cells retain junctions and apical-basal polarity. AVE migration requires the small GTPase Rac1. It is the first in vivo mammalian model of collective cell migration. I will use a combination of mRNA profiling, genetics, ex vivo embryo culture, embryo electroporation, and live imaging to ask: -What are the chemotactic clues and signaling pathways for AVE migration? -What are the behaviors of AVE, VE and epiblast cells during AVE migration? -What are the mechanisms controlling motile cells cohesion and coordination, such as junctions and cytoskeleton rearrangements? -How do mechanical constrains influence morphogenesis? -What is the role of Rho GTPases in AVE migration, EV epithelial stability and junctional tension? It is likely that regenerative, neoplastic and morphogenetic collective movements share common molecular mechanisms.
Promoteur/Supervisor : Prof. Migeotte Isabelle
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Faculté/Faculty : Faculté de Médecine/Faculty of Medicine
Ecole doctorale/Graduate Colleges : Sciences biomédicales et pharmaceutiques/Biomedical and Pharmaceutical Science
Ecole doctorale thématique/Graduate School (French Only):

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